| 出品公司: | Promega |
|---|---|
| 载体名称: | pGL4.82[hRluc/Puro] |
| 质粒类型: | 荧光素酶载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 4684 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
![pGL4.82[hRlucPuro]载体图谱](/uploads/载体图谱/pGL4.82[hRlucPuro]载体图谱.png)
载体简介
载体序列
LOCUS pGL4.82[hRluc Puro] 4684 bp ds-DNA circular SYN 26-7-2013
DEFINITION Promoterless vector for measuring the activity of promoter and
enhancer sequences with a Renilla luciferase assay.
ACCESSION DQ188846
VERSION .
KEYWORDS pGL4.82[hRluc Puro]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4684)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported 2017/2/28 from SnapGene 2.3.2
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4684
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
misc_feature 1..70
/note="MCS"
/note="multiple cloning site"
CDS 100..1035
/codon_start=1
/product="Renilla luciferase"
/note="hRluc"
/note="human codon-optimized"
/protein_id="
"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
polyA_signal 1076..1197
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter 1391..1748
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 1599..1734
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 1779..2378
/codon_start=1
/product="puromycin N-acetyltransferase"
/note="PuroR"
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
polyA_signal 2403..2451
/note="synthetic polyadenylation signal"
rep_origin complement(2778..3366)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3566..4426)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 4531..4579
/note="synthetic polyadenylation signal"
misc_feature 4593..4684
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
1021 aagaacgagc agtaattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga
1081 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
1141 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
1201 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
1261 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtttgc gtattgggcg
1321 ctcttccgct gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt
1381 tagctacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
1441 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
1501 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
1561 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
1621 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
1681 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
1741 tttgcaaaaa gctcgattct tctgacacta gcgccaccat gaccgagtac aagcctaccg
1801 tgcgcctggc cactcgcgat gatgtgcccc gcgccgtccg cactctggcc gccgctttcg
1861 ccgactaccc cgctacccgg cacaccgtgg accccgaccg gcacatcgag cgtgtgacag
1921 agttgcagga gctgttcctg acccgcgtcg ggctggacat cggcaaggtg tgggtagccg
1981 acgacggcgc ggccgtggcc gtgtggacta cccccgagag cgttgaggcc ggcgccgtgt
2041 tcgccgagat cggcccccga atggccgagc tgagcggcag ccgcctggcc gcccagcagc
2101 aaatggaggg cctgcttgcc ccccatcgtc ccaaggagcc tgcctggttt ctggccactg
2161 taggagtgag ccccgaccac cagggcaagg gcttgggcag cgccgtcgtg ttgcccggcg
2221 tagaggccgc cgaacgcgcc ggtgtgcccg cctttctcga aacaagcgca ccaagaaacc
2281 ttccattcta cgagcgcctg ggcttcaccg tgaccgccga tgtcgaggtg cccgagggac
2341 ctaggacctg gtgtatgaca cgaaaacctg gcgcctaatg atctagaacc ggtcatggcc
2401 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gttcgaacta
2461 gatgctgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
2521 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
2581 aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
2641 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
2701 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
2761 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
2821 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
2881 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
2941 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
3001 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
3061 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
3121 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
3181 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
3241 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
3301 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
3361 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
3421 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
3481 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagcggc
3541 cgcaaatgct aaaccactgc agtggttacc agtgcttgat cagtgaggca ccgatctcag
3601 cgatctgcct atttcgttcg tccatagtgg cctgactccc cgtcgtgtag atcactacga
3661 ttcgtgaggg cttaccatca ggccccagcg cagcaatgat gccgcgagag ccgcgttcac
3721 cggcccccga tttgtcagca atgaaccagc cagcagggag ggccgagcga agaagtggtc
3781 ctgctacttt gtccgcctcc atccagtcta tgagctgctg tcgtgatgct agagtaagaa
3841 gttcgccagt gagtagtttc cgaagagttg tggccattgc tactggcatc gtggtatcac
3901 gctcgtcgtt cggtatggct tcgttcaact ctggttccca gcggtcaagc cgggtcacat
3961 gatcacccat attatgaaga aatgcagtca gctccttagg gcctccgatc gttgtcagaa
4021 gtaagttggc cgcggtgttg tcgctcatgg taatggcagc actacacaat tctcttaccg
4081 tcatgccatc cgtaagatgc ttttccgtga ccggcgagta ctcaaccaag tcgttttgtg
4141 agtagtgtat acggcgacca agctgctctt gcccggcgtc tatacgggac aacaccgcgc
4201 cacatagcag tactttgaaa gtgctcatca tcgggaatcg ttcttcgggg cggaaagact
4261 caaggatctt gccgctattg agatccagtt cgatatagcc cactcttgca cccagttgat
4321 cttcagcatc ttttactttc accagcgttt cggggtgtgc aaaaacaggc aagcaaaatg
4381 ccgcaaagaa gggaatgagt gcgacacgaa aatgttggat gctcatactc gtcctttttc
4441 aatattattg aagcatttat cagggttact agtacgtctc tcaaggataa gtaagtaata
4501 ttaaggtacg ggaggtattg gacaggccgc aataaaatat ctttattttc attacatctg
4561 tgtgttggtt ttttgtgtga atcgatagta ctaacatacg ctctccatca aaacaaaacg
4621 aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt
4681 ctct
//
